HPLC Separation of Adenosine, Cordycepin and Adenine on Newcrom AH Column

HPLC.cloud View on hplc.cloud   Due to cordycepin having a very similar structure to adenosine, it has shown to have inhibitive properties on the COVID-19 coronavirus. However, due to their similar structures, the separation of the two sugars can be challenging. Both sugars can be separated isocratically in about six minutes on the Newcrom AH mixed-mode column, which has both hydrophobic and cationic exchange properties. The mobile phase consists of acetonitrile (ACN, MeCN) and water with ammonium formate as a buffer which makes it mass-spec (MS) compatible. It can also be UV detected at 260nm.  


Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm,  MS-compatible mobile phase


Class of Compounds Hydrophilic, Drug, Xanthine, Nucleobase
Analyzing Compounds Adenosine,  Cordycepin, Adenine
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Application Analytes: