Deoxyuridine

CAS Number	951-78-0
Molecular Formula	C9H12N2O5
Molecular Weight	228.204
InChI Key	MXHRCPNRJAMMIM-SHYZEUOFSA-N
LogP	-1.6
Synonyms	2'-DEOXYURIDINE deoxyuridine 951-78-0 Uracil deoxyriboside 2-Deoxyuridine 1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione Deoxyribose uracil Uridine, 2'-deoxy- 2'-Desoxyuridine UNII-W78I7AY22C CCRIS 2832 dURD EINECS 213-455-7 BRN 0024433 2 -Deoxyuridine CHEBI:16450 1-(2-Deoxy-beta-D-erythro-pentofuranoxyl)uracil MFCD00006527 1-(2-Deoxy-beta-D-ribofuranosyl)uracil AK-54658

Applications:

HPLC Separation of Nucleobases (2)

December 6, 2007

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Nucleosides consisting of deoxyribose and a nucleobase cannot be separated on a common C18 column. By using a Primesep 200 mixed-mode column with a cation-exchange mechanism, nucleosides: deoxyuridine, deoxyguanosine, deoxycytidine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and TFA as buffer. UV detection at 270nm.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Deoxyuridine

UV Detection

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Deoxyribo Nucleic Acid

September 24, 2003

View Application

Primesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.