HPLC Method for Deoxyuridine on Chromni™ by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Deoxyuridine

Deoxyuridine (dU) is an nucleoside with the C₉H₁₂N₂O₅ molecular formula. While in living organisms it exists primarily to occasionally become a part of DNA, it’s variants, Idoxuridine and Trifluoride, work as antiviral drugs. If consumed, Deoxyuridine can be toxic.

Deoxyuridine can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

Primesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.