Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method of Adenosine and Deoxyadenosine.
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: adenosine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate (AmFm) buffer. Detection can be achieved with UV 260 nm, mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (,.CAD).
| Column | Newcrom AH, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | AmFm pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 260 nm |
| Class of Compounds |
Nucleatide |
| Analyzing Compounds | Adenosine, Deoxyadenosine |
Application Column
Newcrom AH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsDeoxyadenosine
