HPLC Separation of Adenosine and Deoxyadenosine on Newcrom AH Column

Separation type: Liquid Chromatography Mixed-mode HPLC.cloud View on hplc.cloud High Performance Liquid Chromatography (HPLC) Method of Adenosine and Deoxyadenosine. Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: adenosine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate (AmFm) buffer. Detection can be achieved with UV 260 nm, mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (,.CAD).


Column Newcrom AH, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer  AmFm pH 3.0 - 10 mM
Flow Rate 1.0 ml/min
Detection UV, 260 nm


Class of Compounds Nucleatide
Analyzing Compounds Adenosine,  Deoxyadenosine
Application Analytes: