CAS Number | 78842-13-4 |
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Molecular Formula | C10H12FN5O4 |
Molecular Weight | 285.23 |
InChI Key | UXUZARPLRQRNNX-DXTOWSMRSA-N |
LogP | -0.9 |
Synonyms |
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Applications:
HPLC Separation of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine on Newcrom AH Cloumn
June 1, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. 2′-Deoxy-2′-fluoroguanosine is an antiviral nuceloside that can help the body fight influenza A & B. Deoxyguanosine is one of the four core deoxyribonucleosides that compose DNA. The only difference chemically is the addition of a fluorine atom on the non-Nitrogen ring.
2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be detected in the high UV regime. Using a Primesep SB mixed-mode column and a mobile phase consisting of just water (without a buffer), 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be retained, separated, and analyzed. This analysis method can be UV detected at 252 nm with high resolution. This method is compatible with Mass Spectrometry.
Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | H2O – 100% |
Buffer | No |
Flow Rate | 1.0 ml/min |
Detection | UV, 252 nm |
Class of Compounds |
Nucleatide |
Analyzing Compounds | 2′-Deoxy-2′-fluoroguanosine, Deoxyadenosine |
Application Column
Newcrom AH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsPrimesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsDeoxyguanosine