HPLC Separation of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine on Newcrom AH Cloumn

HPLC Method for Deoxyguanosine, 2'-Deoxy-2'-fluoroguanosine on Newcrom AH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine

Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. 2′-Deoxy-2′-fluoroguanosine is an antiviral nuceloside that can help the body fight influenza A & B. Deoxyguanosine is one of the four core deoxyribonucleosides that compose DNA. The only difference chemically is the addition of a fluorine atom on the non-Nitrogen ring.

2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be detected in the high UV regime. Using a Newcrom AH mixed-mode column and a mobile phase consisting of just water (without a buffer), 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be retained, separated, and analyzed. This analysis method can be UV detected at 252 nm with high resolution. This method is compatible with Mass Spectrometry.

 

Condition
Column Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase H2O – 100%
Buffer  No
Flow Rate 1.0 ml/min
Detection UV, 252 nm

 

Description
Class of Compounds
 Nucleatide
Analyzing Compounds Deoxyguanosine, 2'-Deoxy-2'-fluoroguanosine

 

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
2′-Deoxy-2′-fluoroguanosine
Deoxyguanosine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.