Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. 2′-Deoxy-2′-fluoroguanosine is an antiviral nuceloside that can help the body fight influenza A & B. Deoxyguanosine is one of the four core deoxyribonucleosides that compose DNA. The only difference chemically is the addition of a fluorine atom on the non-Nitrogen ring.
2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be detected in the high UV regime. Using a Newcrom AH mixed-mode column and a mobile phase consisting of just water (without a buffer), 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be retained, separated, and analyzed. This analysis method can be UV detected at 252 nm with high resolution. This method is compatible with Mass Spectrometry.
| Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | H2O – 100% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 252 nm |
| Class of Compounds |
Nucleatide |
| Analyzing Compounds | 2′-Deoxy-2′-fluoroguanosine, Deoxyadenosine |
Application Column
Newcrom AH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Deoxyguanosine
