HPLC Separation of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine on Newcrom AH Cloumn

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of 2'-Deoxy-2'-fluoroguanosine and Deoxyguanosine
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. 2′-Deoxy-2′-fluoroguanosine is an antiviral nuceloside that can help the body fight influenza A & B. Deoxyguanosine is one of the four core deoxyribonucleosides that compose DNA. The only difference chemically is the addition of a fluorine atom on the non-Nitrogen ring. 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be detected in the high UV regime. Using a Primesep SB mixed-mode column and a mobile phase consisting of just water (without a buffer), 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be retained, separated, and analyzed. This analysis method can be UV detected at 252 nm with high resolution. This method is compatible with Mass Spectrometry.

Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase H2O - 100%
Buffer  No
Flow Rate 1.0 ml/min
Detection UV, 252 nm
 

Description

Class of Compounds Nucleatide
Analyzing Compounds 2'-Deoxy-2'-fluoroguanosine,  Deoxyadenosine
 
Application Analytes:
2′-Deoxy-2′-fluoroguanosine
Deoxyguanosine