| CAS Number | 961-07-9 |
|---|---|
| Molecular Formula | C10H13N5O4 |
| Molecular Weight | 267.24 |
| InChI Key | YKBGVTZYEHREMT-KVQBGUIXSA-N |
| LogP | -0.9 |
| Synonyms |
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Applications:
Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+
December 5, 2022
Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine
Condition
| Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | H3PO4, H2SO4, HFGA (Hexafluoroglutaric acid) – 0.2%, |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Peak Retention Time |
Description
| Class of Compounds | Nucleosides |
| Analyzing Compounds | Deoxyguanosine, Guanine and Guanosine |
Application Column
BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
Select optionsGuanine
Guanosine
HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+
December 5, 2022
HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine
Condition
| Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 85% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Peak Retention Time | 9.6, 11.2, 12.8 min |
Description
| Class of Compounds | Nucleosides |
| Analyzing Compounds | Deoxyguanosine, Guanine and Guanosine |
Application Column
BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
Select optionsGuanine
Guanosine
HPLC Separation of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine on Newcrom AH Cloumn
June 1, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. 2′-Deoxy-2′-fluoroguanosine is an antiviral nuceloside that can help the body fight influenza A & B. Deoxyguanosine is one of the four core deoxyribonucleosides that compose DNA. The only difference chemically is the addition of a fluorine atom on the non-Nitrogen ring.
2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be detected in the high UV regime. Using a Newcrom AH mixed-mode column and a mobile phase consisting of just water (without a buffer), 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be retained, separated, and analyzed. This analysis method can be UV detected at 252 nm with high resolution. This method is compatible with Mass Spectrometry.
| Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | H2O – 100% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 252 nm |
| Class of Compounds |
Nucleatide |
| Analyzing Compounds | 2′-Deoxy-2′-fluoroguanosine, Deoxyadenosine |
Application Column
Newcrom AH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Deoxyguanosine
HPLC Separation of Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine on Newcrom AH Column
May 25, 2021
Separation type: Liquid Chromatography Mixed-mode
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.
| Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Nucleoside, Hydrophilic, Ionizable |
| Analyzing Compounds | Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine |
Application Column
Newcrom AH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsDeoxyadenosine
Deoxyguanosine
Guanosine
HPLC Separation of Nucleosides and Deoxynucleosides
July 23, 2012
| Column | Sharc 1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/MeOH |
| Buffer | AmFm, Formic acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsCytidine
Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Guanosine
Thymidine
Uridine
Separation of Deoxyribo Nucleosides
September 24, 2003
Primesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Deoxycytidine
Deoxyguanosine
Deoxyuridine
