HPLC Method for Carbocisteine, Methylparaben sodium, Propylparaben sodium, Propylparaben, Methylparaben on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Carbocisteine, Methylparaben sodium, Propylparaben sodium, Propylparaben, Methylparaben.
Carbocysteine is a medication that reduces the viscosity of mucus making it easier to cough up. Methylparaben and propylparaben are used as preservatives in cosmetics, pharmaceuticals and food. They can be separated in HPLC on a Primesep 100 mixed-mode column by both hydrophobic and ionic properties present on the stationary phase. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.
| Column | Primesep 100 |
| Mobile Phase | Gradient MeCN – 2-50%, 20 min |
| Buffer | H2SO4 – 0.05 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic, Benzoate, Hydroxy Acid, Amino Acid, Hydroxybenzoate, Phenol |
| Analyzing Compounds | Carbocisteine, Methylparaben sodium, Propylparaben sodium, Propylparaben, Methylparaben |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
CarbocisteineMethylparaben
Methylparaben sodium
Propylparaben
Propylparaben sodium
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
