| CAS Number | 76058-33-8 |
|---|---|
| Molecular Formula | C17H19N3O2 |
| Molecular Weight | 297.358 |
| InChI Key | HBRCDTRQDHMTDA-UHFFFAOYSA-N |
| LogP | 4.1 |
| Synonyms |
|
Applications:
UV-Vis Spectrum of Ethyl Red
July 16, 2024
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Separation of Methyl Red and Ethyl Red on Primesep 100 Column
December 22, 2022
High Performance Liquid Chromatography (HPLC) Method for Separation of Methyl Red and Ethyl Red on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Separation of Меthyl Red and Ethyl Red
Methyl Red is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions. Ethyl Red is a magenta-colored, single-charged basic dye. These two basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | Vis, 520 nm |
| Peak Retention Time | 6.19, 7.28 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Меthyl red, Ethyl red |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsMethyl Red
HPLC Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 Column
December 7, 2022
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Pararosaniline (Basic Red 9) is a popular basic magenta dye and part of the triarylmethane family of dyes. Ethyl Red is a similarly-colored, single-charged basic dye. These two basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the visible regime at 520 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Pararosaniline and Ethyl red
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 520 nm |
| Peak Retention Time | 10.82 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Pararosaniline, Ethyl red |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPararosaniline Hydrochloride
HPLC Method for Analysis of Ethyl Red on Primesep 100 Column
December 7, 2022
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl Red on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analyses of Ethyl red
Ethyl red can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 600 nm with high resolution and peak symmetry.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 550 nm |
| Peak Retention Time | 3.82 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Ethyl red |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
HPLC Method for Analysis of Ethyl Red
June 1, 2018
HPLC Method for Ethyl red on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl red
Ethyl red is a pH indicator with C₁₇H₁₉N₃O₂ as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods.
Ethyl red can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/10% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Dye, Hydrophobic |
| Analyzing Compounds | Ethyl red |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
