High Performance Liquid Chromatography (HPLC) Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Pararosaniline (Basic Red 9) is a popular basic magenta dye and part of the triarylmethane family of dyes. Ethyl Red is a similarly-colored, single-charged basic dye. These two basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the visible regime at 520 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Pararosaniline and Ethyl red
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 520 nm |
| Peak Retention Time | 10.82 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Pararosaniline, Ethyl red |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPararosaniline Hydrochloride
