HPLC Method for Ethyl red on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl red
Ethyl red is a pH indicator with C₁₇H₁₉N₃O₂ as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods.
Ethyl red can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 60/10% |
Buffer | H3PO4 – 0.1% |
Flow Rate | 0.5 ml/min |
Detection | UV, 250 nm |
Class of Compounds | Dye, Hydrophobic |
Analyzing Compounds | Ethyl red |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended