| CAS Number | 76058-33-8 |
|---|---|
| Molecular Formula | C17H19N3O2 |
| Molecular Weight | 297.358 |
| InChI Key | HBRCDTRQDHMTDA-UHFFFAOYSA-N |
| LogP | 4.1 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Ethyl Red
July 16, 2024
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Ethyl red check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Separation of Methyl Red and Ethyl Red on Primesep 100 Column
December 22, 2022
High Performance Liquid Chromatography (HPLC) Method for Separation of Methyl Red, Ethyl red on Primesep 100 by SIELC Technologies.
Methyl Red, also known as C.I. Acid Red 2, is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions and yellow at solution with pH above 6.2. It has the chemical formula C15H15N3O2. You can find detailed UV spectra of Methyl Red and information about its various lambda maxima by visiting the following link.
Ethyl red is a pH indicator with C17H19N3O2 as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods. You can find detailed UV spectra of Ethyl Red and information about its various lambda maxima by visiting the following link.
Methyl Red, Ethyl red can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | Vis, 520 nm |
| Peak Retention Time | 6.19, 7.28 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Methyl Red, Ethyl red |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Methyl Red
HPLC Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 Column
December 7, 2022
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 by SIELC Technologies.
Pararosaniline (Basic Red 9) is a popular basic magenta dye and part of the triarylmethane family of dyes with the chemical formula C19H17N3. It is a free base version of pararosaniline hydrochloride. Primarily, it is used to dye synthetic materials, to detect sulfur dioxide, and as an antischistosomal. You can find detailed UV spectra of Pararosaniline and information about its various lambda maxima by visiting the following link.
Ethyl Red is a pH indicator with C₁₇H₁₉N₃O₂ as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods. You can find detailed UV spectra of Pararosaniline Hydrochloride, Ethyl red and information about its various lambda maxima by visiting the following link.
These two basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the visible regime at 520 nm.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 520 nm |
| Peak Retention Time | 10.82 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Pararosaniline Hydrochloride, Ethyl red |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Pararosaniline Hydrochloride
HPLC Method for Analysis of Ethyl Red on Primesep 100 Column
December 7, 2022
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl red on Primesep 100 by SIELC Technologies.
Ethyl Red is a pH indicator with C₁₇H₁₉N₃O₂ as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods. You can find detailed UV spectra of Ethyl red and information about its various lambda maxima by visiting the following link.
Ethyl red can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 600 nm with high resolution and peak symmetry.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 550 nm |
| Peak Retention Time | 3.82 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Ethyl red |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Analysis of Ethyl Red
June 1, 2018
HPLC Method for Ethyl red on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl red
Ethyl red is a pH indicator with C₁₇H₁₉N₃O₂ as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods. You can find detailed UV spectra of Ethyl red and information about its various lambda maxima by visiting the following link.
Ethyl red can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/10% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Dye, Hydrophobic |
| Analyzing Compounds | Ethyl red |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
