HPLC Method for Analysis of Ethyl Red on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl Red on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Ethyl Red on Primesep 100 Column
HPLC Method for Analysis of Ethyl Red on Primesep 100 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analyses of Ethyl red

Ethyl red can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer.  This analysis method can be UV detected at 600 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 80/20%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 550 nm
Peak Retention Time3.82 min

Description

Class of CompoundsDyes
Analyzing CompoundsEthyl red

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Ethyl red
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.