| CAS Number | 56-81-5 |
|---|---|
| Molecular Formula | C3H8O3 |
| Molecular Weight | 92.095 |
| InChI Key | PEDCQBHIVMGVHV-UHFFFAOYSA-N |
| LogP | -1.76 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Glycerol on Primesep AP Column
August 7, 2025
HPLC Method for Glycerol on Primesep AP by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycerol
Glycerol is an organic compound with the molecular formula C3H8O3.
Properties:
Appearance: Typically a colorless, odorless, and syrupy liquid.
Molecular weight: ~92.09 g/mol
Solubility: Soluble in Water.
Uses: Used in many industries and for many products including skin care, sweetener, dry skin solutions, soap, moisturizers, pharmaceuticals, humectant and food.
Glycerol can be retained and analyzed using the Primesep AP stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD or RI.
| Column | Primesep AP, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 90% |
| Buffer | Formic Acid – 0.5% |
| Detection | ELSD or RI |
| Class of Compounds | Simple Triol |
| Analyzing Compounds | Glycerol |
Application Column
Primesep AP
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
RI Detection
HPLC Method For Simultaneous Quantification of Sugar Alcohols (Polyols) on Primesep AP
September 9, 2021
Separation type: Liquid Chromatography HILIC
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, Glycerol, Erythritol, Xylitol, Sorbitol.
| Column | Primesep AP, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 75/25% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm, RI |
| Class of Compounds |
Drug, Basic, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Ethylene glycol, Glycerol, Erythritol , Xylitol, Sorbitol |
Application Column
Primesep AP
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsEthylene Glycol
Glycerol
Sorbitol
Xylitol
HPLC Derivatization and Quantitative Analysis of Glycerol
July 16, 2010
Glycerol is a polyol hydrophilic compound which is used in pharmaceutical formulations. It is also a byproduct of biodisel production through transesterification. Glycerol has three hydroxyl groups, making it a very polar compound. Method using periodate/acetylacteone derivatization was developed using UV. Method is fast and shows good linearity within explored range of concentrations. This general HPLC approach can be used for precise quantitation of glycerol.
Application Column
Primesep N
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
