HPLC Derivatization and Quantitative Analysis of Glycerol

Glycerol is a polyol hydrophilic compound which is used in pharmaceutical formulations. It is also a byproduct of biodisel production through transesterification. Glycerol has three hydroxyl groups, making it a very polar compound. Method using periodate/acetylacteone derivatization was developed using UV. Method is fast and shows good linearity within explored range of concentrations. This general HPLC approach can be used for precise quantitation of glycerol.

SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:

Application Detection:
UV Detection