HPLC Method for Analysis of Glycerol on Primesep AP Column

HPLC Method for Glycerol on Primesep AP by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycerol

Glycerol is an organic compound with the molecular formula C3H8O3.

Properties:
Appearance: Typically a colorless, odorless, and syrupy liquid.

Molecular weight: ~92.09 g/mol

Solubility: Soluble in Water.

Uses: Used in many industries and for many products including skin care, sweetener, dry skin solutions, soap, moisturizers, pharmaceuticals, humectant and food.

Glycerol can be retained and analyzed using the Primesep AP stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD or RI.

ColumnPrimesep AP, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 90%
BufferFormic Acid – 0.5%
DetectionELSD or RI

Class of CompoundsSimple Triol
Analyzing CompoundsGlycerol

Application Column

Primesep AP

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Glycerol
Application Detection:
ELSD Detection
RI Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.