HPLC Method for Glycerol, Xylitol, Sorbitol, Erythritol, Ethylene Glycol on Primesep AP by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycerol, Xylitol, Sorbitol, Erythritol, Ethylene Glycol.

Ethylene glycol is an organic compound with the formula (CH₂OH)₂. It has a variety of uses, but it is primarily found as an antifreeze agent. Those coolants are often used in automobiles and air-conditioning systems. In the plastic industry, ethylene glycol is a precursor to polyester fibers and resins.

Glycerol is an organic compound with the molecular formula C₃H₈O₃. It is a humectant, solvent, and sweetener that used in various industries including skin care, hygiene products, pharmaceuticals, and food. It is said to improve lubrication and smoothness in products. Historically, before ethylene glycol took over, glycerol was used as a antifreeze.

Erythritol is a naturally accruing sugar alcohol with the chemical formula C₄H₁₀O₄. It is is typically used as a sweetener and flavor enhancer in a large variety of foods and bevarages, including but not limited to tea, juice blends, soft drinks, cookies, biscuits, sugar-free chewing gum, and confections. It does lead to a noticeably different texture in baked products if used as a sugar replacement.

Xylitol is a sugar alcohol with the chemical formula C₅H₁₂O₅. It is primarily used as a sugar supplement in confections, toothpaste, and drugs. It is considered a safe option for those with diabetes as it has negligible effects on blood sugar. It is said to produce a cooling sensation when interacting with moisture, which makes it an interesting additive to textiles.

Sorbitol is a sugar alcohol with the chemical formula C₆H₁₄O₆. It is used primarily as a sugar substitute, but it also provides some dietary energy. Medically, it can be used as a laxative. In modern cosmetics, it is also used as a humectant and thickener.

Glycerol, Xylitol, Sorbitol, Erythritol, Ethylene Glycol can be retained and analyzed using the Primesep AP stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

Xylitol is a polyalcohol which is used as a sweetener for people with diabetes. Xylitol is naturally found in the fibers of fruits and vegetables. It is a very highly polar, non-ionizable molecule. Xylitol can be well retained on the Primesep S HILIC column. It has a relatively high water concentration in the mobile phase that helps to dissolve xylitol at high acetonitrile HILIC applications. Polar surface on the Primesep S column provides well controlled retention time and can be used as a robust method for analysis of various sugar-lie molecules.

Xylose is a monosaccharide which can be found in wood. Xylitol is a sugar-like, open chain polyalcohol. Xylitol and xylose were separated on the Primesep S HILIC/mixed-mode column. Both compounds are retained by a single HILIC mechanism. This method is compatible with ELSD, CAD, and RI detection and can be used for analysis of various monosaccharides in complex mixtures.