HPLC Analysis on Xylose and Xylitol on Primesep S Column

Xylose is a monosaccharide which can be found in wood. Xylitol is a sugar-like, open chain polyalcohol. Xylitol and xylose were separated on the Primesep S HILIC/mixed-mode column. Both compounds are retained by a single HILIC mechanism. This method is compatible with ELSD, CAD, and RI detection and can be used for analysis of various monosaccharides in complex mixtures.


Column Primesep S, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 80/20%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELSD



Class of Compounds
Sugar, Hydrophilic, Ionizable
Analyzing Compounds  Xylose, Xylitol


Application Column

Primesep S

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.