HPLC Analysis on Xylose and Xylitol on Primesep S Column

Xylose is a monosaccharide which can be found in wood. Xylitol is a sugar-like, open chain polyalcohol. Xylitol and xylose were separated on the Primesep S HILIC/mixed-mode column. Both compounds are retained by a single HILIC mechanism. This method is compatible with ELSD, CAD, and RI detection and can be used for analysis of various monosaccharides in complex mixtures.


Column Primesep S, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 80/20%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Sugar, Hydrophilic, Ionizable
Analyzing Compounds  Xylose, Xylitol
Application Analytes: