HPLC Analysis of Xylitol on Primesep S Column

Xylitol is a polyalcohol which is used as a sweetener for people with diabetes. Xylitol is naturally found in the fibers of fruits and vegetables. It is a very highly polar, non-ionizable molecule. Xylitol can be well retained on the Primesep S HILIC column. It has a relatively high water concentration in the mobile phase that helps to dissolve xylitol at high acetonitrile HILIC applications. Polar surface on the Primesep S column provides well controlled retention time and can be used as a robust method for analysis of various sugar-lie molecules.


Column Primesep S, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 80/20%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELSD



Class of Compounds
Sugar, Hydrophilic, Ionizable
Analyzing Compounds Xylitol


Application Column

Primesep S

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.