HPLC Analysis of Xylitol on Primesep S Column

Xylitol is a polyalcohol which is used as a sweetener for people with diabetes. Xylitol is naturally found in the fibers of fruits and vegetables. It is a very highly polar, non-ionizable molecule. Xylitol can be well retained on the Primesep S HILIC column. It has a relatively high water concentration in the mobile phase that helps to dissolve xylitol at high acetonitrile HILIC applications. Polar surface on the Primesep S column provides well controlled retention time and can be used as a robust method for analysis of various sugar-lie molecules.

Condition

Column Primesep S, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 80/20%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Sugar, Hydrophilic, Ionizable
Analyzing Compounds Xylitol
 
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Application Analytes:
Xylitol

Application Detection:
ELSD/MS Detection