HPLC Method  For Simultaneous Quantification of Sugar Alcohols (Polyols) on Primesep AP

Separation type: Liquid Chromatography HILIC

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, Glycerol, Erythritol, Xylitol, Sorbitol.


Column Primesep AP, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 75/25%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 200 nm, RI



Class of Compounds
Drug, Basic, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds Ethylene glycol, Glycerol, Erythritol , Xylitol, Sorbitol


Application Column

Primesep AP

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Ethylene Glycol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.