Primesep B is a mixed-mode analytical column. Its design, which includes basic ion-pairing groups embedded on long ligand chains, is specifically tailored for direct plasma analysis. The column features a C12 carbon chain and basic groups with a pKa value of 10.

– It does not retain high-molecular weight components found in plasma.
– It effectively retains both hydrophobic and acidic components.
– All mobile phases are compatible with LC-MS and preparative chromatography, operating within a pH range of 1.5 to 4.