HPLC Method for Analysis of Meclizine on Primesep B Column

Meclizine is a histamine H1 antagonist and a synthetic piperazine. It is used to prevent and treat motion sickness, vertigo, and nausea during pregnancy. The side effects that may occur include drowsiness, dry mouth, and tiredness. The method is UV compatible and can be used as a general approach for analyzing similar compounds.



Column Primesep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.2 %
Flow Rate 1.0 ml/min
Detection UV, 232 nm



Class of Compounds
Drug, Antihistamine, Hydrophobic
Analyzing Compounds Meclizine


Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.