HPLC Determination of Acrylic Acid on Primesep B Column

Acrylic acid, or propenoic acid, is used in a production of various copolymers for manufacturing of diverse range products. It consists of a vinyl group and a carboxylic acid. Acrylic acid can be retained on a reverse-phase (RP) mixed-mode Primesep B column in under 5 minutes. Primesep B is a reverse-phase column with embedded basic ion-pairing groups.



Column Primesep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.1 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm



Class of Compounds
Acid, Hydrophilic, Ionizable, Carboxylic acid
Analyzing Compounds Acrylic Acid


Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Acrylic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.