This application on Primesep B demonstrates the easy tuning of methods developed on Primesep columns. Aromatic carboxylic acids are easily separated and retention times can be shifted by simply changing the mobile phase modifier. Switching between formic, trifluoroacetic acid, phosphoric, and perchloric acids in a constant ratio of acetonitrile and water changes elution times, which is not possible on reversed-phase C18 columns. This effect is due to both the change in mobile phase pH and the counter ion that interacts with the basic functional group on the column. Mass spec compatible mobile phases of water, acetonitrile (MeCN, ACN) and, formic or trifluoracetic acid (TFA) can be chosen for LC/MS applications.
| Column | Primesep B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | TFA, H3PO4, HClO4, FA |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Carboxylic Acids |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCarboxylic Acids
