Aromatic Acids



    Separation of Aromatic Carboxylic Acids by Mixed-Mode HPLC

    May 5, 2005

    This application on Primesep B demonstrates the easy tuning of methods developed on Primesep columns. Aromatic carboxylic acids are easily separated and retention times can be shifted by simply changing the mobile phase modifier. Switching between formic, trifluoroacetic acid, phosphoric, and perchloric acids in a constant ratio of acetonitrile and water changes elution times, which is not possible on reversed-phase C18 columns. This effect is due to both the change in mobile phase pH and the counter ion that interacts with the basic functional group on the column. Mass spec compatible mobile phases of water, acetonitrile (MeCN, ACN) and, formic or trifluoracetic acid (TFA) can be chosen for LC/MS applications.


    Column Primesep B, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN
    Buffer TFA, H3PO4, HClO4, FA
    Flow Rate 1.0 ml/min
    Detection UV, 250 nm



    Class of Compounds
    Acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Carboxylic Acids


    Application Column

    Primesep B

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Aromatic Acids
    Carboxylic Acids

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.