HPLC Method for Analysis of Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate on Primesep B Column

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate on Primesep B Column_1474

High Performance Liquid Chromatography (HPLC) Method for Analysis of Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate

Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate is an ultra violet hindered amine light stabilizer commonly used in plastics production and in the packaging and manufacturing of pharmaceutical products. Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate can be retained and analyzed on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 210 nm with high resolution and peak symmetry.



Column Primesep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm



Class of Compounds
Analyzing Compounds Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.