Quinine and quinidine are diastereomers. Quinine is an alkaloid with fever-reducing analgetic and anti-inflammatory properties. Both compounds have the same structure, and the only difference is optical position at two centers. These two diastereomers were separated on achiral mixed-mode cation-exclusion Primesep B column. No separation is observed on traditional achiral reversed-phase column. This examples shows that even diastereomers can be separated on mixed-mode HPLC columns.
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsQuinidine
Quinine
