HPLC Determination of Thymol and Carvacrol on Primesep B Column

HPLC Method for Thymol, Carvacrol on Primesep B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol and Carvacrol.

Thymol (2-isopropyl-5-methylphenol) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties.

Carvacrol (5-Isopropyl-2-methylphenol), also known as cymophenol,  is the main component of the essential oils of the Lamiaceae family of plant. It is used in food and cosmetics for it’s taste and aroma.

Thymol and Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.

Condition
Column Primesep B, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 45/55%
Buffer H3PO4 – 0.1%
Flow Rate 0.5 ml/min
Detection UV 200 nm

Description
Class of Compounds Phenol, Hydrophobic
Analyzing Compounds Thymol, Carvacrol

 

Application Column

Primesep B

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Carvacrol
Thymol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.