HPLC Determination of Thymol and Carvacrol on Primesep B Column

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol and Carvacrol.

Thymol (2-isopropyl-5-methylphenol) and Carvacrol (5-Isopropyl-2-methylphenol) are the main components of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. Thymol and Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.



Column Primesep B, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 45/55%
Buffer H3PO4 – 0.1%
Flow Rate 0.5 ml/min
Detection 200 nm


Class of Compounds Phenol, Hydrophobic
Analyzing Compounds Thymol, carvacrol


Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.