| CAS Number | 499-75-2 |
|---|---|
| Molecular Formula | C10H14O |
| Molecular Weight | 150.22 |
| InChI Key | RECUKUPTGUEGMW-UHFFFAOYSA-N |
| LogP | 3.1 |
| Synonyms |
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Applications:
HPLC Determination of Carvacrol on Primesep B Column
July 29, 2024
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Carvacrol
Carvacrol (5-Isopropyl-2-methylphenol) is the main component of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.
| Column | [Model] |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | 200 nm |
| Class of Compounds | Phenol, Hydrophobic |
| Analyzing Compounds | Carvacrol |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Determination of Thymol and Carvacrol on Primesep B Column
July 14, 2021
HPLC Method for Thymol, Carvacrol on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol and Carvacrol.
Thymol (2-isopropyl-5-methylphenol) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties.
Carvacrol (5-Isopropyl-2-methylphenol), also known as cymophenol, is the main component of the essential oils of the Lamiaceae family of plant. It is used in food and cosmetics for it’s taste and aroma.
Thymol and Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Phenol, Hydrophobic |
| Analyzing Compounds | Thymol, Carvacrol |
Application Column
Primesep B
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Thymol
