Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Carvacrol
Carvacrol (5-Isopropyl-2-methylphenol) is the main component of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.
| Column | [Model] |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | 200 nm |
| Class of Compounds | Phenol, Hydrophobic |
| Analyzing Compounds | Carvacrol |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
CarvacrolApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
