HPLC Determination of Carvacrol on Primesep B Column

HPLC Method for Carvacrol on Primesep B by SIELC Technologies

HPLC Method for Analysis of Carvacrol on Primesep B Column

Carvacrol, also known as 5-Isopropyl-2-methylphenol, is the main component of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. It has an odor similar to oregano. You can find detailed UV spectra of Carvacrol and information about its various lambda maxima by visiting the following link.

Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.

Condition
Column [Model]
Mobile Phase MeCN/H2O – 45/55%
Buffer H3PO4 – 0.1%
Flow Rate 0.5 ml/min
Detection 200 nm

Description
Class of Compounds Phenol, Hydrophobic
Analyzing Compounds Carvacrol

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Carvacrol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.