| CAS Number | 89-83-8 |
|---|---|
| Molecular Formula | C10H14O |
| Molecular Weight | 150.221 |
| InChI Key | MGSRCZKZVOBKFT-UHFFFAOYSA-N |
| LogP | 3.30 |
| Synonyms |
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Applications:
HPLC Determination of Thymol and Carvacrol on Primesep B Column
July 14, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol and Carvacrol.
Thymol (2-isopropyl-5-methylphenol) and Carvacrol (5-Isopropyl-2-methylphenol) are the main components of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. Thymol and Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.
| Column | Primesep B, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | 200 nm |
| Class of Compounds | Phenol, Hydrophobic |
| Analyzing Compounds | Thymol, carvacrol |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsThymol
HPLC Determination of Thymol on Newcrom R1
June 14, 2021
Separation type: Liquid Chromatography Reverse Phase.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol.
Thymol (2-isopropyl-5-methylphenol) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties. Thymol can be retained in HPLC using Newcrom R1 column with the simple isocratic mobile phase consisting of methanol (MeOH) and water. The analysis method can be UV detected at 210nm.
| Column | Newcrom R1, 2.1×100 mm, 5 µm, 100A |
| Mobile Phase | MeOH – 70% |
| Buffer | No |
| Flow Rate | 0.2 ml/min |
| Detection | 210 nm |
| Class of Compounds | Phenol, Hydrophobic |
| Analyzing Compounds | Thymol |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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Separation of Thymol on Newcrom R1 HPLC column
February 16, 2018
Thymol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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