HPLC Determination of Thymol on Newcrom R1

Separation type: Liquid Chromatography Reverse Phase.

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol.

Thymol (2-isopropyl-5-methylphenol) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties. Thymol can be retained in HPLC using Newcrom R1 column with the simple isocratic mobile phase consisting of methanol (MeOH) and water. The analysis method can be UV detected at 210nm.



Column Newcrom R1, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeOH – 70%
Buffer No
Flow Rate 0.2 ml/min
Detection 210 nm


Class of Compounds Phenol, Hydrophobic
Analyzing Compounds Thymol


Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.