HPLC Determination of Thymol on Newcrom R1

Separation type: Liquid Chromatography Reverse Phase.

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol.

Thymol (2-isopropyl-5-methylphenol) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties. Thymol can be retained in HPLC using Newcrom R1 column with the simple isocratic mobile phase consisting of methanol (MeOH) and water. The analysis method can be UV detected at 210nm.



Column Newcrom R1, 2.1x100 mm, 5 µm, 100A
Mobile Phase MeOH - 70%
Buffer No
Flow Rate 0.2 ml/min
Detection 210 nm


Class of Compounds Phenol, Hydrophobic
Analyzing Compounds Thymol
Application Analytes: