HPLC Method for Thymol on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol.
Thymol (2-isopropyl-5-methylphenol) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties.
Thymol can be retained in HPLC using Newcrom R1 column with the simple isocratic mobile phase consisting of methanol (MeOH) and water. The analysis method can be UV detected at 210nm.
| Column | Newcrom R1, 2.1 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeOH – 70% |
| Buffer | No |
| Flow Rate | 0.2 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Phenol, Hydrophobic |
| Analyzing Compounds | Thymol |
Application Column
Newcrom R1
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
ThymolApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
