CAS Number | 499-75-2 |
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Molecular Formula | C10H14O |
Molecular Weight | 150.22 |
InChI Key | RECUKUPTGUEGMW-UHFFFAOYSA-N |
LogP | 3.1 |
Synonyms |
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Applications:
HPLC Determination of Carvacrol on Primesep B Column
July 29, 2024
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Carvacrol
Carvacrol (5-Isopropyl-2-methylphenol) is the main component of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.
Column | [Model] |
Mobile Phase | MeCN/H2O – 45/55% |
Buffer | H3PO4 – 0.1% |
Flow Rate | 0.5 ml/min |
Detection | 200 nm |
Class of Compounds | Phenol, Hydrophobic |
Analyzing Compounds | Carvacrol |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Determination of Thymol and Carvacrol on Primesep B Column
July 14, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol and Carvacrol.
Thymol (2-isopropyl-5-methylphenol) and Carvacrol (5-Isopropyl-2-methylphenol) are the main components of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. Thymol and Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.
Column | Primesep B, 3.2×100 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 45/55% |
Buffer | H3PO4 – 0.1% |
Flow Rate | 0.5 ml/min |
Detection | 200 nm |
Class of Compounds | Phenol, Hydrophobic |
Analyzing Compounds | Thymol, carvacrol |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsThymol