Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Drotaverin (No-spa)
Drotaverine (known under the brand name No-Spa in Europe) is an antispasmodic commonly encountered in the hospital setting to increase cervical dilation during childbirth. There have been issues with the drug being counterfeited or smuggled into countries that have yet to approve it, so having an HPLC method to detect the drug and seperate it from impurities and other unwanted compounds is a must for the pharmaceutical community.
Drotaverine can be detected in the high UV regime. Using a Primesep B reverse-phase column and a mobile phase consisting of water and an acetonitrile (MeCN) gradient with a sulfuric acid (H2SO4) buffer, drotaverine can be retained, separated (from impurities and other unwanted compounds). This analysis method can be UV detected at 360 nm.
|Primesep B, 3.2×100 mm, 5 µm, 100A
|Gradient MeCN – 10-50%, 10 min
|H2SO4 – 0.1%
|UV 245, 305, 360 nm
|Class of Compounds
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.Select options