HPLC Method For Analysis of Drotaverine (No-spa) on Primesep B Column

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Drotaverin (No-spa)

Drotaverine (known under the brand name No-Spa in Europe) is an antispasmodic commonly encountered in the hospital setting to increase cervical dilation during childbirth. There have been issues with the drug being counterfeited or smuggled into countries that have yet to approve it, so having an HPLC method to detect the drug and seperate it from impurities and other unwanted compounds is a must for the pharmaceutical community.
Drotaverine can be detected in the high UV regime. Using a Primesep B reverse-phase column and a mobile phase consisting of water and an acetonitrile (MeCN) gradient with a sulfuric acid (H2SO4) buffer, drotaverine can be retained, separated (from impurities and other unwanted compounds). This analysis method can be UV detected at 360 nm.



Column Primesep B, 3.2×100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-50%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 0.5 ml/min
Detection UV 245, 305, 360  nm


Class of Compounds Drug
Analyzing Compounds Drotaverin


Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.