HPLC Method For Analysis of Drotaverine (No-spa) on Primesep B Column

Separation type: Liquid Chromatography Mixed-mode HPLC.cloud View on hplc.cloud High Performance Liquid Chromatography (HPLC) Method for Analysis of Drotaverin (No-spa) Drotaverine (known under the brand name No-Spa in Europe) is an antispasmodic commonly encountered in the hospital setting to increase cervical dilation during childbirth. There have been issues with the drug being counterfeited or smuggled into countries that have yet to approve it, so having an HPLC method to detect the drug and seperate it from impurities and other unwanted compounds is a must for the pharmaceutical community. Drotaverine can be detected in the high UV regime. Using a Primesep B reverse-phase column and a mobile phase consisting of water and an acetonitrile (MeCN) gradient with a sulfuric acid (H2SO4) buffer, drotaverine can be retained, separated (from impurities and other unwanted compounds). This analysis method can be UV detected at 360 nm.  


Column Primesep B, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-50%, 10 min
Buffer H2SO4 - 0.1%
Flow Rate 0.5 ml/min
Detection UV 245, 305, 360  nm


Class of Compounds Drug
Analyzing Compounds Drotaverin
Application Analytes: