HPLC Method for Separation of Ketoconazole and Potassium Sorbate on Primesep B by SIELC Technologies
Ketoconazole, also known as Nizoral, is an antifungal compound with the chemical formula C26H28Cl2N4O4. It is used to treat dandruff and is commonly available as a shampoo. It is applied to skin to treat other fungal infections. Ketoconazole also has antiandrogen and antiglucocorticoid properties. It works through interfering with the fungal synthesis of ergosterol and other enzymes. You can find detailed UV spectra of Ketoconazole and information about its various lambda maxima by visiting the following link.
Potassium Sorbate is a popular preservative with the chemical formula C6H7KO2. It is used in a wide variety of foods, wines, and personal-care products. It works through inhibiting molds, yeasts, and various other microbes. You can find detailed UV spectra of Potassium Sorbate and information about its various lambda maxima by visiting the following link.
Potassium sorbate, Sodium sorbate, Ketoconazole can be retained, analyzed, and separated on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.
Condition
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 10-50%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200, 250 nm |
| Peak Retention Time | 6.31, 8.12 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Potassium sorbate, Sodium sorbate, Ketoconazole |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Potassium sorbate
Sodium sorbate
