HPLC Method for Separation of Ketoconazole and Potassium Sorbate on Primesep B Column

HPLC Method for Separation of Ketoconazole and Potassium Sorbate on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Ketoconazole and Potassium Sorbate on Primesep B Column by SIELC Technologies
HPLC Method for Separation of Ketoconazole and Potassium Sorbate on Primesep B by SIELC Technologies

Ketoconazole, also known as Nizoral, is an antifungal compound used to treat dandruff and is commonly available as a shampoo. Ketoconazole can be retained, analyzed, and separated from Potassium sorbate  on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Ketoconazole and Potassium Sorbate

Condition

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 10-50%, 10 min
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200, 250 nm
Peak Retention Time6.31, 8.12 min

Description

Class of CompoundsDrug
Analyzing CompoundsPotassium sorbate, Sodium sorbate, Ketoconazole

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ketoconazole
Potassium sorbate
Sodium sorbate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.