Separation of Pyrazines by Mixed-Mode HPLC

This application on Primesep B demonstrates the easy tuning of methods developed on Primesep columns. The pyrizines, 5-formyl-1H-pyrrole-3-carboxylic acid and pyrrole-2-carbaldehyde, are easily separated and peak order can be reversed by simply changing the mobile phase modifier from trifluoroacetic acid to acetic acid. This effect is due to both the change in mobile phase pH and the counter ion that interacts with the basic functional group on the column. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) or acetic acid (HOAc).

Application Analytes:
5-Formyl-1H-pyrrole-3-carboxylic Acid
Pyrrole-2-carbaldehyde

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep B

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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