Separation of Pyrazines by Mixed-Mode HPLC

This application on Primesep B demonstrates the easy tuning of methods developed on Primesep columns. The pyrizines, 5-formyl-1H-pyrrole-3-carboxylic acid and pyrrole-2-carbaldehyde, are easily separated and peak order can be reversed by simply changing the mobile phase modifier from trifluoroacetic acid to acetic acid. This effect is due to both the change in mobile phase pH and the counter ion that interacts with the basic functional group on the column. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) or acetic acid (HOAc).

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
5-Formyl-1H-pyrrole-3-carboxylic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.