HPLC Determination of β‑Hydroxy-β-methylbutyric Acid on Primesep B Column

HPLC Determination of β‑Hydroxy-β-methylbutyric Acid

Beta-Hydroxy beta-Methylbutyric acid (HMB) is used as an over-the-counter dietary supplement, which has been shown to increase lean body mass and muscle size. Another use for it is in to promote wound healing and to prevent the loss of muscle in individuals suffering from age-related muscle loss. HMB can be retained on a Primesep B mixed-mode column in in 3 minutes using low Acetonitrile (ACN) concentration in the mobile phase (10%) with 0.2% Phosphoric acid buffer. UV detection 200nm.



Column Primesep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.2 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm



Class of Compounds
Acid, Hydrophilic,  Ionizable, Normal human metabolite, Dietary supplement
Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB)




Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
β‑Hydroxy-β-methylbutyric Acid (HMB)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.