| CAS Number | 625-08-1 |
|---|---|
| Molecular Formula | C5H10O3 |
| Molecular Weight | 118.13 |
| InChI Key | AXFYFNCPONWUHW-UHFFFAOYSA-N |
| LogP | -0.3 |
| Synonyms |
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Applications:
HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid on Primesep 100 Colimn
September 20, 2019
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the
essential dietary branched chain amino acid leucine.
Condition
Column Primesep 100, 4.6×150 mm, 5 µm, 100A Mobile Phase MeCN – 5% Buffer H2SO4 – 0.05 % Flow Rate 1.0 ml/min Detection UV, 210 nm
Description
Class of Compounds
Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA) Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA)
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsβ‑Hydroxy-β-methylbutyric Acid (HMB)
HPLC Determination of β‑Hydroxy-β-methylbutyric Acid on Primesep B Column
September 13, 2019
Beta-Hydroxy beta-Methylbutyric acid (HMB) is used as an over-the-counter dietary supplement, which has been shown to increase lean body mass and muscle size. Another use for it is in to promote wound healing and to prevent the loss of muscle in individuals suffering from age-related muscle loss. HMB can be retained on a Primesep B mixed-mode column in in 3 minutes using low Acetonitrile (ACN) concentration in the mobile phase (10%) with 0.2% Phosphoric acid buffer. UV detection 200nm.
| Column | Primesep B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.2 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement |
| Analyzing Compounds | β‑Hydroxy-β-methylbutyric (HMB) |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
HPLC Separation of β‑Hydroxy-β-methylbutyric Acid, 2-Hydroxyisocaproic Acid and Leucine on Primesep 100 Column
September 13, 2019
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the
essential dietary branched chain amino acid leucine.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 5%, 3 min hold, Gradient MeCN 5-30% 12 min |
| Buffer | H2SO4 – 0.05 % 3 min hold, GradientH2SO4 0.05-3% 12 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA) |
| Analyzing Compounds | β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA), Leucine |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsD-Leucine
Leucine
β‑Hydroxy-β-methylbutyric Acid (HMB)
