2-Hydroxyisocaproic Acid (HICA)

CAS Number498-36-2
Molecular FormulaC6H12O3
Molecular Weight132.16
InChI KeyLVRFTAZAXQPQHI-UHFFFAOYSA-N
LogP0.9
Synonyms
  • 2-Hydroxy-4-methylpentanoic acid
  • 498-36-2
  • 2-Hydroxy-4-methylvaleric acid
  • DL-leucic acid
  • 2-Hydroxyisocaproic acid
  • Leucic acid
  • alpha-Hydroxyisocaproic acid
  • leucinic acid
  • 2-HYDROXY-4-METHYL-PENTANOIC ACID
  • Pentanoic acid, 2-hydroxy-4-methyl-
  • DL-2-Hydroxyisocaproic acid
  • d-alpha-hydroxyisocaproic acid
  • DL-alpha-Hydroxyisocaproic acid
  • 10303-64-7
  • DL-2-Hydroxy-4-methylvaleric acid
  • 2-hydroxyisohexanoic acid
  • CHEBI:59783
  • LVRFTAZAXQPQHI-UHFFFAOYSA-N
  • MFCD00004246
  • 2-Hydroxyisocaproate
  • alpha-Hydroxyisocaproate
  • 2-Hydroxy-4-methylpentanoate
  • (+-)-Leucinic acid
  • Leucate
  • DL-Leucate
  • (2S)-2-Hydroxy-4-methyl-pentanoic acid
  • a-Hydroxyisocaproate
  • EINECS 207-860-8
  • EINECS 233-677-8
  • HICA
  • 2-Hydroxyisohexanoate
  • a-Hydroxy-iso-caproate
  • DL-a-Hydroxyisocaproate
  • PubChem22723
  • a-Hydroxyisocaproic acid
  • DL-2-Hydroxyisocaproate
  • AC1L3OTG
  • alpha-Hydroxy-iso-caproate
  • a-Hydroxy-iso-caproic acid
  • bmse000338
  • DL-alpha-Hydroxyisocaproate
  • AC1Q1P3I
  • DL-a-Hydroxyisocaproic acid
  • 2-hydroxy-4-methyl-Valerate
  • SCHEMBL185354
  • alpha-Hydroxy-iso-caproic acid
  • 2-Hydroxyisocaproic acid, 99%
  • CHEMBL3247392
  • CTK8D8669
  • DL-2-Hydroxy-4-methylpentanoate
  • KS-00000YQG
  • LVRFTAZAXQPQHI-UHFFFAOYSA-
  • 2-hydroxy-4-methyl-Valeric acid
  • 4-methyl-2-oxidanyl-pentanoic acid
  • 2-Hydroxy-4-methylpentanoic acid
  • #BBL003540
  • DL-2-Hydroxy-4-methylpentanoic acid
  • LMFA01050381

Applications:

HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid on Primesep 100 Colimn

September 20, 2019

HPLC Method for β‑Hydroxy-β-methylbutyric Acid (HMB), 2-Hydroxyisocaproic Acid (HICA) on Primesep 100 by SIELC Technologies

HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid_1182

 High Performance Liquid Chromatography (HPLC) Method for Analysis of β‑Hydroxy-β-methylbutyric Acid (HMB), 2-Hydroxyisocaproic Acid (HICA) 

Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the
essential dietary branched chain amino acid leucine.

β‑Hydroxy-β-methylbutyric Acid (HMB), 2-Hydroxyisocaproic Acid (HICA) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric Acid buffer. Detection is performed using UV.

 

Condition

Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 5%
Buffer H2SO4 – 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Acid, Hydrophilic,  Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA)
Analyzing Compounds β‑Hydroxy-β-methylbutyric Acid (HMB), 2-Hydroxyisocaproic Acid (HICA)

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
2-Hydroxyisocaproic Acid (HICA)
β‑Hydroxy-β-methylbutyric Acid (HMB)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of β‑Hydroxy-β-methylbutyric Acid, 2-Hydroxyisocaproic Acid and Leucine on Primesep 100 Column

September 13, 2019

HPLC Method for β‑Hydroxy-β-methylbutyric Acid (HMB), D-Leucine, Leucine, 2-Hydroxyisocaproic Acid (HICA) on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of β‑Hydroxy-β-methylbutyric Acid (HMB), D-Leucine, Leucine, 2-Hydroxyisocaproic Acid (HICA) 

Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the
essential dietary branched chain amino acid leucine.

β‑Hydroxy-β-methylbutyric Acid (HMB), D-Leucine, Leucine, 2-Hydroxyisocaproic Acid (HICA) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

 

Condition

Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 5%, 3 min hold, Gradient MeCN 5-30% 12 min
Buffer H2SO4 – 0.05 % 3 min hold, GradientH2SO4 0.05-3% 12 min
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Acid, Hydrophilic,  Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA)
Analyzing Compounds β‑Hydroxy-β-methylbutyric Acid (HMB), D-Leucine, Leucine, 2-Hydroxyisocaproic Acid (HICA)

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
2-Hydroxyisocaproic Acid (HICA)
D-Leucine
Leucine
β‑Hydroxy-β-methylbutyric Acid (HMB)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.