| CAS Number | 625-08-1 |
|---|---|
| Molecular Formula | C5H10O3 |
| Molecular Weight | 118.13 |
| InChI Key | AXFYFNCPONWUHW-UHFFFAOYSA-N |
| LogP | -0.3 |
| Synonyms |
|
Applications:
HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid on Primesep 100 Colimn
September 20, 2019
HPLC Method for β‑Hydroxy-β-methylbutyric Acid (HMB), 2-Hydroxyisocaproic Acid (HICA) on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of β‑Hydroxy-β-methylbutyric Acid (HMB), 2-Hydroxyisocaproic Acid (HICA)
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the
essential dietary branched chain amino acid leucine.β‑Hydroxy-β-methylbutyric Acid (HMB), 2-Hydroxyisocaproic Acid (HICA) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric Acid buffer. Detection is performed using UV.
Condition
Column Primesep 100 Mobile Phase MeCN – 5% Buffer H2SO4 – 0.05 % Flow Rate 1.0 ml/min Detection UV, 210 nm
Description
Class of Compounds
Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA) Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA)
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
β‑Hydroxy-β-methylbutyric Acid (HMB)
HPLC Determination of β‑Hydroxy-β-methylbutyric Acid on Primesep B Column
September 13, 2019
HPLC Method for β‑Hydroxy-β-methylbutyric Acid (HMB) on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of β‑Hydroxy-β-methylbutyric Acid (HMB)
Beta-Hydroxy beta-Methylbutyric acid (HMB) is used as an over-the-counter dietary supplement, which has been shown to increase lean body mass and muscle size. Another use for it is in to promote wound healing and to prevent the loss of muscle in individuals suffering from age-related muscle loss. HMB can be retained on a Primesep B mixed-mode column in in 3 minutes using low Acetonitrile (ACN) concentration in the mobile phase (10%) with 0.2% Phosphoric acid buffer. UV detection 200nm.
| Column | Primesep B |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.2 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement |
| Analyzing Compounds | β‑Hydroxy-β-methylbutyric (HMB) |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Separation of β‑Hydroxy-β-methylbutyric Acid, 2-Hydroxyisocaproic Acid and Leucine on Primesep 100 Column
September 13, 2019
HPLC Method for β‑Hydroxy-β-methylbutyric Acid (HMB), D-Leucine, Leucine, 2-Hydroxyisocaproic Acid (HICA) on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of β‑Hydroxy-β-methylbutyric Acid (HMB), D-Leucine, Leucine, 2-Hydroxyisocaproic Acid (HICA)
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the
essential dietary branched chain amino acid leucine.β‑Hydroxy-β-methylbutyric Acid (HMB), D-Leucine, Leucine, 2-Hydroxyisocaproic Acid (HICA) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100 |
| Mobile Phase | MeCN – 5%, 3 min hold, Gradient MeCN 5-30% 12 min |
| Buffer | H2SO4 – 0.05 % 3 min hold, GradientH2SO4 0.05-3% 12 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA) |
| Analyzing Compounds | β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA), Leucine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
D-Leucine
Leucine
β‑Hydroxy-β-methylbutyric Acid (HMB)
