Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.
| Column | Primesep B, 3.2×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 90/10% |
| Buffer | |
| Flow Rate | 0.5 ml/min |
| Detection | UV 205 |
| Class of Compounds | Vitamins, Hydrophilic, Ionizable |
| Analyzing Compounds | Vitamin E, Vitamin E Acetate |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsVitamin E Acetate
