| CAS Number | 59-02-9 |
|---|---|
| Molecular Formula | C29H50O2 |
| Molecular Weight | 430.717 |
| InChI Key | GVJHHUAWPYXKBD-IEOSBIPESA-N |
| LogP | 10.2 |
| Synonyms |
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Applications:
HPLC MS Method for Analysis of Vitamin E ((±)-α-Tocopherol) and Vitamin E Acetate (α-Tocopheryl Acetate) on Lipak Column
December 13, 2024
HPLC Method for Vitamin E, Vitamin E Acetate on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin E, Vitamin E Acetate
(±)-α-Tocopherol is a form of vitamin E, a fat-soluble antioxidant that protects cell membranes from oxidative damage by neutralizing free radicals. Widely found in dietary sources like nuts and vegetable oils, it plays a critical role in maintaining skin health, immune function, and overall cellular protection. α-Tocopheryl acetate, on the other hand, is a stable esterified form of α-tocopherol. Commonly used in dietary supplements and skincare products, it is converted to active α-tocopherol in the body, offering a prolonged shelf life and enhanced stability in formulations.
Vitamin E, Vitamin E Acetate can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), ammonium formate and formic acid as a buffer. Detection is achieved using UV 292 nm or MS.
| Column | Lipak, 3.2 x 150 mm, 3 µm, 100 A |
| Mobile Phase | Gradient MeOH/H2O – 90/10 – 100/0% in 10 min, 5 min hold |
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 292 nm ESI MS SIM 431 +, 473+ |
| Class of Compounds | Fat Soluble Vitamins |
| Analyzing Compounds | Vitamin E, Vitamin E Acetate |
Application Column
Vitamin E Acetate
LC MS Detection
HPLC Separation of Vitamin E and Vitamin E Acetate on Primesep B Column
March 27, 2004
Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.
| Column | Primesep B, 3.2×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 90/10% |
| Buffer | |
| Flow Rate | 0.5 ml/min |
| Detection | UV 205 |
| Class of Compounds | Vitamins, Hydrophilic, Ionizable |
| Analyzing Compounds | Vitamin E, Vitamin E Acetate |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsVitamin E Acetate
