CAS Number | 58-95-7 |
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Molecular Formula | C31H52O3 |
Molecular Weight | 472.754 |
InChI Key | ZAKOWWREFLAJOT-CEFNRUSXSA-N |
LogP | 10.3 |
Synonyms |
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Applications:
HPLC MS Method for Analysis of Vitamin E ((±)-α-Tocopherol) and Vitamin E Acetate (α-Tocopheryl Acetate) on Lipak Column
December 13, 2024
HPLC Method for Vitamin E, Vitamin E Acetate on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin E, Vitamin E Acetate
(±)-α-Tocopherol is a form of vitamin E, a fat-soluble antioxidant that protects cell membranes from oxidative damage by neutralizing free radicals. Widely found in dietary sources like nuts and vegetable oils, it plays a critical role in maintaining skin health, immune function, and overall cellular protection. α-Tocopheryl acetate, on the other hand, is a stable esterified form of α-tocopherol. Commonly used in dietary supplements and skincare products, it is converted to active α-tocopherol in the body, offering a prolonged shelf life and enhanced stability in formulations.
Vitamin E, Vitamin E Acetate can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), ammonium formate and formic acid as a buffer. Detection is achieved using UV 292 nm or MS.
Column | Lipak, 3.2 x 150 mm, 3 µm, 100 A |
Mobile Phase | Gradient MeOH/H2O – 90/10 – 100/0% in 10 min, 5 min hold |
Buffer | AmFm– 10 mM, FA – 0.05% |
Flow Rate | 0.5 ml/min |
Detection | UV 292 nm ESI MS SIM 431 +, 473+ |
Class of Compounds | Fat Soluble Vitamins |
Analyzing Compounds | Vitamin E, Vitamin E Acetate |
Application Column
Vitamin E Acetate
LC MS Detection
HPLC Analysis of Ascorbic Acid, Retinol (Vitamin A) and Vitamin E acetate in ACE Serum Active Ingredients
October 14, 2010
Mixed-mode HPLC columns allow for separation of compounds with drastically different properties. In this application, highly hydrophilic ascorbic acid is retained and separated from highly hydrophobic retinol (Vitamin A) and Vitamin E Acetate. Ascorbic acid is retained by anion-exchange mechanism and hydrophobic compounds are retained by reversed-phase mechanism. Compounds are monitored by UV detection. Mobile phase is compatible with LC/MS and method can be used to monitor basic and acidic compounds in bio-fluids (serum, blood, urine, saliva, etc.)
Retinol (Vitamin A) and Vitamin E Acetate
Column | Primesep SB , 3.0×50 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O |
Buffer | Acetic Acid |
Flow Rate | 0.5 ml/min |
Detection | UV 270, 325 |
Class of Compounds |
Acid, Vitamin B₆, Hydrophobic, Ionizable |
Analyzing Compounds | Ascorbic acid (Vitamin C), Retinol (Vitamin A) and Vitamin E Acetate |
Application Column
Primesep SB
Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Retinol (Vitamin A)
Vitamin E Acetate
HPLC Separation of Vitamin E and Vitamin E Acetate on Primesep B Column
March 27, 2004
Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.
Column | Primesep B, 3.2×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 90/10% |
Buffer | |
Flow Rate | 0.5 ml/min |
Detection | UV 205 |
Class of Compounds | Vitamins, Hydrophilic, Ionizable |
Analyzing Compounds | Vitamin E, Vitamin E Acetate |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsVitamin E Acetate