Vitamin E Acetate

Vitamin E Acetate structural formula

CAS Number58-95-7
Molecular FormulaC31H52O3
Molecular Weight472.754
InChI KeyZAKOWWREFLAJOT-CEFNRUSXSA-N
LogP10.3
Synonyms
  • D-alpha-Tocopheryl acetate
  • (2R)-2,5,7,8-Tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-3,4-dihydro-2H-1-benzopyran-6-yl acetate
  • 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-, acetate, (2R)-
  • 58-95-7
  • 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-, acetate, (2R)-
  • (+)-α-Tocopherol acetate
  • (+)-α-Tocopheryl acetate
  • (2R,4'R,8'R)-α-Tocopherol acetate
  • (2R,4'R,8'R)-α-Tocopheryl acetate
  • (R,R,R)-α-Tocopheryl acetate
  • 2,5,7,8-Tetramethyl-2-(4,8,12-trimethyltridecyl)-6-chromanol acetate
  • 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-, acetate, [2R-[2R*(4R*,8R*)]]-
  • 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-, 6-acetate, (2R)-
  • 6-Chromanol, 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-, acetate, (+)-
  • Acetate d'α-tocopheryle
  • acetato de α-tocoferilo
  • Combinal E
  • Copherol 12250
  • Copherol 1250
  • Covitol
  • Covitol 1100
  • Covitol 1360
  • Covitol 700
  • Covitol 700WD
  • d-Vitamin E acetate
  • D-α-Tocopherol acetate
  • D-α-TOCOPHERYL ACETATE
  • E 230 Clear
  • E-Toplex
  • E-Vicotrat
  • Evipherol
  • Lutavit E 50
  • Nanotopes
  • Natur-E granulate
  • Spondyvit
  • Tinoderm E
  • TOCOPHEROL ACETATE, (+)-α-
  • TOCOPHERYL ACETATE
  • TOCOPHERYL ACETATE, D-α-
  • TOCOPHERYL D-α ACETATE
  • Tokoferol acetate
  • Vitamin E 230 Clear
  • Vitamin Eα acetate
  • α-Tocopherylacetat
  • Alfacol
  • 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-, acetate, (2R-(2*(4R*,8R*)))-
  • Contopheron
  • 6-Cromanol, 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-, acetate, (+)-
  • 3,4-Dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl acetate, (2R-(2*(4R*,8R*)))-
  • Ecofrol
  • EINECS 200-405-4
  • Ephynal acetate
  • Epsilan-M
  • E-Ferol
  • 2,5,7,8-Tetramethyl-2-(4,8,12-trimethyltridecyl)-6-cromanyl acetate, (+)-
  • Tocopherex
  • (+)-alpha-Tocopherol acetate
  • d-alpha-Tocopherol acetate
  • alpha-Tocopherol acetate, (2R,4'R,8'R)-
  • (+)-alpha-Tocopheryl acetate
  • (R,R,R)-alpha-Tocopheryl acetate
  • (2R,4'R,8'R)-alpha-Tocopheryl acetate
  • Tocophrin
  • Tofaxin
  • Endo E Dompe
  • Vitamin Ealpha acetate
  • Vitamin E acetate, d-
  • UNII-A7E6112E4N
  • Simmyungsaengmosu
  • 12741-00-3
  • 1406-70-8
  • 26243-95-8

Applications:

HPLC MS Method for Analysis of Vitamin E ((±)-α-Tocopherol) and Vitamin E Acetate (α-Tocopheryl Acetate) on Lipak Column

December 13, 2024

HPLC Method for Vitamin E, Vitamin E Acetate on Lipak by SIELC Technologies

HPLC Method for Analysis of Vitamin E and Vitamin E Acetate on Lipak Column by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin E, Vitamin E Acetate

(±)-α-Tocopherol is a form of vitamin E, a fat-soluble antioxidant that protects cell membranes from oxidative damage by neutralizing free radicals. Widely found in dietary sources like nuts and vegetable oils, it plays a critical role in maintaining skin health, immune function, and overall cellular protection. α-Tocopheryl acetate, on the other hand, is a stable esterified form of α-tocopherol. Commonly used in dietary supplements and skincare products, it is converted to active α-tocopherol in the body, offering a prolonged shelf life and enhanced stability in formulations.

Vitamin E, Vitamin E Acetate can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), ammonium formate and formic acid as a buffer. Detection is achieved using UV 292 nm or MS.

ColumnLipak, 3.2 x 150 mm, 3 µm, 100 A
Mobile PhaseGradient MeOH/H2O – 90/10 – 100/0% in 10 min, 5 min hold
BufferAmFm– 10 mM, FA – 0.05%
Flow Rate0.5 ml/min
DetectionUV 292 nm
ESI MS SIM 431 +, 473+

Class of CompoundsFat Soluble Vitamins
Analyzing CompoundsVitamin E, Vitamin E Acetate

Application Column

Lipak

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A

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Application Analytes:
Vitamin E
Vitamin E Acetate

Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Ascorbic Acid, Retinol (Vitamin A) and Vitamin E acetate in ACE Serum Active Ingredients

October 14, 2010


Mixed-mode HPLC columns allow for separation of compounds with drastically different properties. In this application, highly hydrophilic ascorbic acid is retained and separated from highly hydrophobic retinol (Vitamin A) and Vitamin E Acetate. Ascorbic acid is retained by anion-exchange mechanism and hydrophobic compounds are retained by reversed-phase mechanism. Compounds are monitored by UV detection. Mobile phase is compatible with LC/MS and method can be used to monitor basic and acidic compounds in bio-fluids (serum, blood, urine, saliva, etc.)
Retinol (Vitamin A)  and Vitamin E Acetate

Condition

Column Primesep  SB , 3.0×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Acetic Acid
Flow Rate 0.5 ml/min
Detection UV 270, 325

 

Description

Class of Compounds
Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Ascorbic acid (Vitamin C), Retinol (Vitamin A) and Vitamin E Acetate

Application Column

Primesep SB

Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Ascorbic Acid
Retinol (Vitamin A)
Vitamin E Acetate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Vitamin E and Vitamin E Acetate on Primesep B Column

March 27, 2004

Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.

Condition

Column Primesep B, 3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 90/10%
Buffer
Flow Rate 0.5 ml/min
Detection UV 205

Description

Class of Compounds Vitamins, Hydrophilic, Ionizable
Analyzing Compounds Vitamin E,  Vitamin E Acetate

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Vitamin E
Vitamin E Acetate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.