Mixed-mode HPLC columns allow for separation of compounds with drastically different properties. In this application, highly hydrophilic ascorbic acid is retained and separated from highly hydrophobic retinol (Vitamin A) and Vitamin E Acetate. Ascorbic acid is retained by anion-exchange mechanism and hydrophobic compounds are retained by reversed-phase mechanism. Compounds are monitored by UV detection. Mobile phase is compatible with LC/MS and method can be used to monitor basic and acidic compounds in bio-fluids (serum, blood, urine, saliva, etc.)
Retinol (Vitamin A) and Vitamin E Acetate
| Column | Primesep SB , 3.0×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV 270, 325 |
| Class of Compounds |
Acid, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Ascorbic acid (Vitamin C), Retinol (Vitamin A) and Vitamin E Acetate |
Application Column
Primesep SB
Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
Ascorbic AcidRetinol (Vitamin A)
Vitamin E Acetate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
