Retinol (Vitamin A)

Retinol structural formula

CAS Number68-26-8
Molecular FormulaC20H30O
Molecular Weight286.460
InChI KeyFPIPGXGPPPQFEQ-OVSJKPMPSA-N
LogP5.68
Synonyms
  • Retinol
  • 68-26-8
  • All-trans retinol
  • Vitamin A
  • 4-06-00-04133
  • (all-E)-3,7-Dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4,6,8-nonatetraen-1-ol
  • (all-E)-3,7-Dimethyl-9-(2,6,6-trimethyl-1-cyclohexene-1-yl)-2,4,6,8-nonatetraen-1-ol
  • 2,4,6,8-Nonatetraen-1-ol, 3,7-dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-, (all-E)-
  • Agiolan
  • Agoncal
  • Alcovit A
  • all-trans-Retinol
  • all-trans-Retinyl alcohol
  • all-trans-Vitamin A
  • all-trans-Vitamin A alcohol
  • all-trans-Vitamin A1
  • Alphalin
  • Alphasterol
  • A-Mulsal
  • Anatola
  • Anatola A
  • Anti-Infective vitamin
  • Antixerophthalmic vitamin
  • Apostavit
  • Aquasol A Parenteral
  • Aquasynth
  • A-Vi-Pel
  • A-Vitan
  • Axerophthol
  • Bentavit A
  • Biosterol
  • Cylasphere
  • Disatabs Tabs
  • Dohyfral A
  • Epiteliol
  • Hi-A-Vita
  • Lard Factor
  • Myvpack
  • Nio-A-Let
  • NSC 122759
  • Oleovitamin a
  • Ophthalamin
  • Plivit A
  • Prepalin
  • Retinol 50C
  • Retinol, all-trans-
  • Retinyl A
  • Tegosphere VitA
  • Testavol
  • Thalasphere
  • trans-Retinol
  • trans-Vitamin A alcohol
  • Veroftal
  • Vi-Alpha
  • Vi-Dom-A
  • Vitamin A alcohol
  • Vitamin A alcohol, all-trans-
  • Vitamin A1 alcohol
  • Vitamin A1 alcohol, all-trans-
  • Vitamin A1, all-trans-
  • Vitavel A
  • Vogan-Neu
  • ?-Retinol
  • Axerophtholum
  • BRN 0403040
  • 3,7-Dimethyl-9-(2,6,6-trimethyl-1-cyclchexen-1-yl)-2,4,6,8-nonatetraen-1-ol
  • 3,7-Dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4,6,8-nonatetraen-1-ol, (all-E)-
  • EINECS 200-683-7
  • Homagenets Aoral
  • Retrovitamin A
  • Sehkraft A
  • Super A
  • Vi-alpha; Vi-alpha
  • Vitamin A1 alcohol, all trans
  • Vitamine A
  • Vitaminum A
  • Vitavel-A
  • Wachstumsvitamin
  • Antixerophthalmisches Vitamin
  • Retinolo
  • Retinolum
  • UNII-G2SH0XKK91
  • (2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohex-1-en-1-yl)nona-2,4,6,8-tetraen-1-ol
  • Chocola A
  • all-trans-retinol
  • b-Retinol
  • beta-Retinol
  • 13123-33-6
  • 17104-91-5
  • 5979-23-7

Applications:

Separation of Retinol on Newcrom R1 HPLC column

February 16, 2018
Separation of Retinol on Newcrom C18 HPLC column

Retinol, also known as Vitamin A1, is a vitamin essential for life. It is often supplemented, although that may be dangerous. Retinol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Retinol (Vitamin A)
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.

HPLC Analysis of Ascorbic Acid, Retinol (Vitamin A) and Vitamin E acetate in ACE Serum Active Ingredients

October 14, 2010


Mixed-mode HPLC columns allow for separation of compounds with drastically different properties. In this application, highly hydrophilic ascorbic acid is retained and separated from highly hydrophobic retinol (Vitamin A) and Vitamin E Acetate. Ascorbic acid is retained by anion-exchange mechanism and hydrophobic compounds are retained by reversed-phase mechanism. Compounds are monitored by UV detection. Mobile phase is compatible with LC/MS and method can be used to monitor basic and acidic compounds in bio-fluids (serum, blood, urine, saliva, etc.)
Retinol (Vitamin A)  and Vitamin E Acetate

Condition
Column Primesep  SB , 3.0×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Acetic Acid
Flow Rate 0.5 ml/min
Detection UV 270, 325

 

Description
Class of Compounds
 Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Ascorbic acid (Vitamin C), Retinol (Vitamin A) and Vitamin E Acetate

Application Column

Primesep SB

Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ascorbic Acid
Retinol (Vitamin A)
Vitamin E Acetate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.