| CAS Number | 127-47-9 |
|---|---|
| Molecular Formula | C22H32O2 |
| Molecular Weight | 328.497 |
| InChI Key | QGNJRVVDBSJHIZ-QHLGVNSISA-N |
| LogP | 7.48 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Vitamin A Acetate
February 18, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Retinol acetate check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Retinol and Synthetic Derivatives Analysis on Lipak Column
November 25, 2024
HPLC Method for Retinol (Vitamin A), Retinol acetate, Retinol palmitate on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Retinol (Vitamin A), Retinol acetate, Retinol palmitate
Retinol, also known as vitamin A1, with the chemical formula C20H30O. It is primarily taken as a daily supplement for a variety of purposes including but not limited to upkeep of skin, improvement of immune functions, and reproductive development. You can find detailed UV spectra of Retinol and information about its various lambda maxima by visiting the following link.
Retinyl Acetate is a synthetic retinyl ester with the chemical formula C22H32O2. It has a variety of uses in dietary supplements and cosmetic products. It is more stable against heat, oxygen and light compared to regular Retinol. You can find detailed UV spectra of Retinyl Acetate and information about its various lambda maxima by visiting the following link.
Retinyl Palmitate is an ester of Vitamin A and Palmitic Acid with the chemical formula C36H60O2. It is the most prescribed version of Vitamin A supplements and is used primarily to treat Vitamin A deficiency. It is also added to low-fat milk and other dairy products as a substitute to the vitamins lost during fat removal. You can find detailed UV spectra of Retinyl Palmitate and information about its various lambda maxima by visiting the following link.
and its synthetic derivatives are widely studied for their impact on skin health, cellular regeneration, and their role in treating various skin conditions like acne, wrinkles, and hyperpigmentation.
Retinol (Vitamin A), Retinol acetate, Retinol palmitate can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), Ethanol (EtOH), ammonium formate and formic acid as a buffer. Detection is achieved using 325 nm
| Column | Lipak, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeOH/H2O/EtOH –80/20/0 – 80/0/20% 10 min, 8 min hold |
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 0.5 ml/min |
| Detection | 325 nm |
| Class of Compounds | Retinoids |
| Analyzing Compounds | Retinol (Vitamin A), Retinol acetate, Retinol palmitate |
Application Column
Lipak
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Retinol acetate
Retinol palmitate
Separation of Retinol acetate on Newcrom R1 HPLC column
February 16, 2018
Retinol acetate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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