Retinol acetate

Retinol acetate structural formula

CAS Number127-47-9
Molecular FormulaC22H32O2
Molecular Weight328.497
InChI KeyQGNJRVVDBSJHIZ-QHLGVNSISA-N
LogP7.48
Synonyms
  • Retinol acetate
  • O15-Acetylretinol
  • Retinol, O15-acetyl-
  • 127-47-9
  • all-trans-Retinyl acetate
  • Retinol, acetate
  • Acetate de retinyle
  • ACETATE, RETINOL
  • acetato de retinilo
  • all-trans-Retinol acetate
  • all-trans-Vitamin A acetate
  • NSC 122045
  • NSC 122760
  • Retinol, acetate, all-trans-
  • retinyl acetate
  • Retinylacetat
  • trans-Retinol acetate
  • trans-Retinyl acetate
  • Vitamin A acetate
  • Vitamin A1 acetate
  • all-trans-Retinylacetate
  • BRN 1915439
  • Crystalets
  • Davitan A 650
  • EINECS 204-844-2
  • Myvak
  • Myvax
  • Retinyl acetate, all-trans-
  • Vitamin A ester
  • UNII-3LE3D9D6OY
  • O(15)-acetylretinol
  • Vitamin A alcohol acetate
  • 11098-51-4
  • 13116-20-6
  • 7095-40-1
  • 80180-27-4

Applications:

HPLC Method for Analysis of Retinol and Synthetic Derivatives Analysis on Lipak Column

November 25, 2024

HPLC Method for Retinol (Vitamin A), Retinol acetate, Retinol palmitate on Lipak by SIELC Technologies

HPLC Method for Analysis of Phospholipids on Lipak Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Retinol (Vitamin A), Retinol acetate, Retinol palmitate

Retinol, also known as vitamin A1, and its synthetic derivatives are widely studied for their impact on skin health, cellular regeneration, and their role in treating various skin conditions like acne, wrinkles, and hyperpigmentation.

Retinol (Vitamin A), Retinol acetate, Retinol palmitate can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), Ethanol (EtOH), ammonium formate and formic acid as a buffer. Detection is achieved using 325 nm

ColumnLipak, 3.2 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeOH/H2O/EtOH –80/20/0 – 80/0/40% 10 min, 8 min hold
BufferAmFm– 10 mM, FA – 0.05%
Flow Rate0.5 ml/min
Detection325 nm

Class of Compounds
Retinoids
Analyzing CompoundsRetinol (Vitamin A), Retinol acetate, Retinol palmitate

Application Column

Lipak

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Retinol (Vitamin A)
Retinol acetate
Retinol palmitate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Retinol acetate on Newcrom R1 HPLC column

February 16, 2018
Separation of Retinol acetate on Newcrom C18 HPLC column

Retinol acetate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Retinol acetate
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.