HPLC Method for Analysis of Retinol and Synthetic Derivatives Analysis on Lipak Column

HPLC Method for Retinol (Vitamin A), Retinol acetate, Retinol palmitate on Lipak by SIELC Technologies

HPLC Method for Analysis of Phospholipids on Lipak Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Retinol (Vitamin A), Retinol acetate, Retinol palmitate

Retinol, also known as vitamin A1, and its synthetic derivatives are widely studied for their impact on skin health, cellular regeneration, and their role in treating various skin conditions like acne, wrinkles, and hyperpigmentation.

Retinol (Vitamin A), Retinol acetate, Retinol palmitate can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), Ethanol (EtOH), ammonium formate and formic acid as a buffer. Detection is achieved using 325 nm

ColumnLipak, 3.2 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeOH/H2O/EtOH –80/20/0 – 80/0/40% 10 min, 8 min hold
BufferAmFm– 10 mM, FA – 0.05%
Flow Rate0.5 ml/min
Detection325 nm

Class of Compounds
Retinoids
Analyzing CompoundsRetinol (Vitamin A), Retinol acetate, Retinol palmitate

Application Column

Lipak

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Retinol (Vitamin A)
Retinol acetate
Retinol palmitate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.