HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid on Primesep B Column

HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid_1182

2-Hydroxyethyl salicylate is a drug used as analgesic agents for the treatment of mild to moderate pain.

 

Condition

Column Primesep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 25/75%
Buffer H2SO4 – 0.5 %
Flow Rate 1.0 ml/min
Detection UV, 238 nm

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Carbocyclic, Benzoate, Hydroxy Acid, Hydroxybenzoate, Phenol
Analyzing Compounds 2-Hydroxyethyl Salicylate, Salicylic Acid

 

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Hydroxyethyl salicylate
Salicylic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.