HPLC Method for 2-Hydroxyethyl salicylate, Salicylic acid on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Hydroxyethyl salicylate, Salicylic acid
2-Hydroxyethyl salicylate is a drug used as analgesic agents for the treatment of mild to moderate pain.
2-Hydroxyethyl salicylate, Salicylic acid can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep B |
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H2SO4 – 0.5 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 238 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Carbocyclic, Benzoate, Hydroxy Acid, Hydroxybenzoate, Phenol |
| Analyzing Compounds | 2-Hydroxyethyl salicylate, Salicylic acid |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
2-Hydroxyethyl salicylateSalicylic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
